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How to open an excel file from labview?

First of all you added onto the end of a 5 year old thread -- not good. You are luck anybody saw this.
Second, if I understand you correctly, your basic approach is flawed. It is never a good idea to have a file open in two place.

What exactly are you trying to do?
What program is writing to the file?

Mike...

PS: Rather than answering here it might be better to start a new thread and reference this discussion.

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